Spectrophotometric quantification of paracetamol and tramadol hydrochloride by chemometric calibration methods

The results of UV spectrophotometric analysis were analysed using partial least squares (PLS) and principal component regression (PCR) techniques to allow simultaneous evaluation tramadol hydrochloride (TRA) p-acetaminophen (PAR) in tablets. A calibration set 16 mixtures, each containing PAR TRA various amounts, was created a 24-full fractional design. absorbance data for the obtained between 215?280 nm (?? = 0.1 nm). Subsequently, concentration sets used generate PCR PLS calibrations. ratio spectra- first derivative method devised as solution same problem compare outcomes chemometric methods experiment. After proposed shown be accurate testing validation samples, they analysing commercial tablet samples. analytical showed that can alternative high-performance liquid chromatography (HPLC). Correlation coefficients determined working range 6?36 g.mL?1 4?22 TRA. limits detection quantification calculated 0.9104 ?g.mL?1 3.0347 ?g.mL?1, respectively. test analyses form are agreement with one-way ANOVA confidence interval 95%. This study shows method, models based on measurements very useful straightforward quantitative resolution two-component pharmaceutical preparation, requiring little sample preparation time analysis.